Isolate Human Regulatory T Cells the Easy Way!
If you are looking for a high yield and purity of human regulatory T cells, with the flexibility to adapt your protocol, try the new Dynal® CD4+CD25+ Treg Kit (Prod.No 113.23) for:
- High yield & purity of Treg cells
- Improve suppressive effect by depleting CD45RA+ cells (optional)
- Bead-free cells avoid potential cell activation
- Isolated cells are suitable for all downstream applications
- Isolate from MNC (up to 1 x 109 total MNC) per kit
You can adapt your protocol depending on your requirements :Choice 1 – do you want CD25+CD45RA+ cells in your sample?CD45 mAb is supplied separately in the kit so you decide if you add it to the antibody mix: + CD45 mAb : remove these cells for enriched Treg cells and increased T cell suppression. - CD45 mAb : keep all CD4+CD25+ T cells for your downstream application Choice 2 – do you want bead-free cells ?Bead-free cells – detach the beads so Treg cells can be used in flow cytometry, suppressive assays or any other downstream assays.Cells-with-beads – for molecular studies.This easy, reliable method uses a standard tube with a magnet, with no need for columns. You just need to decide what to do with the isolated cells!